Journal: Technology and Health Care

Article Title: A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

doi: 10.3233/THC-218050

Figure Lengend Snippet: RT-PCR primer sequences for target genes and reference gene

Article Snippet: Hydroxyapatite bioceramics (synthetized by Beijing Yihuajian science and Trade Co., Ltd., China), endothelial cell culture medium ECM (1001, ScienCell, USA), Potassium phosphate buffer (PBS) (SH30256 01B, Hyclone, USA), clean bench (sw-cj-ifd, Suzhou Antai, China), low-speed centrifuge (sc3614, Zhongjia, China), inverted optical microscope (CKX41, Olympus, Japan), cell incubator (Heracell 150i, Thermo Scientific, USA) and CCK-8 solution (Kga317, Jiangsu Kaiji, China), plate reader (Multiscan MK3, Thermo Fisher Scientific, USA ), Ang1 and Tie2 antibody (251671, Beijing Zen bio, China), Ang2 antibody (24613-1-ap, Proteintech, USA), RIPA buffer (P0013B, Shanghai Beyotime, China), secondary antibody (111-035-003, Jackson ImmunoResearch, USA), luminescent solution (wbkls0500, Millipore, USA), BCA Protein Assay Kit (PA115, Beijing Tiangen, China), PVDF membrane (ipvh00010, Millipore, USA), TRIzol reagent (1556018, Invitrogen, USA), SYBR GREEN qPCR Super Mix (C11733046, Invitrogen, USA), fluorescent PCR (1725274, ABI, USA).

Techniques: Sequencing

Journal: Technology and Health Care

Article Title: A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

doi: 10.3233/THC-218050

Figure Lengend Snippet: Ang2 and Tie2 were up-regulated by hydroxyapatite bioceramic extract (** P < 0.01, *** P < 0.001).

Article Snippet: Hydroxyapatite bioceramics (synthetized by Beijing Yihuajian science and Trade Co., Ltd., China), endothelial cell culture medium ECM (1001, ScienCell, USA), Potassium phosphate buffer (PBS) (SH30256 01B, Hyclone, USA), clean bench (sw-cj-ifd, Suzhou Antai, China), low-speed centrifuge (sc3614, Zhongjia, China), inverted optical microscope (CKX41, Olympus, Japan), cell incubator (Heracell 150i, Thermo Scientific, USA) and CCK-8 solution (Kga317, Jiangsu Kaiji, China), plate reader (Multiscan MK3, Thermo Fisher Scientific, USA ), Ang1 and Tie2 antibody (251671, Beijing Zen bio, China), Ang2 antibody (24613-1-ap, Proteintech, USA), RIPA buffer (P0013B, Shanghai Beyotime, China), secondary antibody (111-035-003, Jackson ImmunoResearch, USA), luminescent solution (wbkls0500, Millipore, USA), BCA Protein Assay Kit (PA115, Beijing Tiangen, China), PVDF membrane (ipvh00010, Millipore, USA), TRIzol reagent (1556018, Invitrogen, USA), SYBR GREEN qPCR Super Mix (C11733046, Invitrogen, USA), fluorescent PCR (1725274, ABI, USA).

Techniques:

Journal: Technology and Health Care

Article Title: A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

doi: 10.3233/THC-218050

Figure Lengend Snippet: The transcriptional level of ANG2 and TIE2 were up-regulated by hydroxyapatite bioceramic extract (** P < 0.01, *** P < 0.001).

Article Snippet: Hydroxyapatite bioceramics (synthetized by Beijing Yihuajian science and Trade Co., Ltd., China), endothelial cell culture medium ECM (1001, ScienCell, USA), Potassium phosphate buffer (PBS) (SH30256 01B, Hyclone, USA), clean bench (sw-cj-ifd, Suzhou Antai, China), low-speed centrifuge (sc3614, Zhongjia, China), inverted optical microscope (CKX41, Olympus, Japan), cell incubator (Heracell 150i, Thermo Scientific, USA) and CCK-8 solution (Kga317, Jiangsu Kaiji, China), plate reader (Multiscan MK3, Thermo Fisher Scientific, USA ), Ang1 and Tie2 antibody (251671, Beijing Zen bio, China), Ang2 antibody (24613-1-ap, Proteintech, USA), RIPA buffer (P0013B, Shanghai Beyotime, China), secondary antibody (111-035-003, Jackson ImmunoResearch, USA), luminescent solution (wbkls0500, Millipore, USA), BCA Protein Assay Kit (PA115, Beijing Tiangen, China), PVDF membrane (ipvh00010, Millipore, USA), TRIzol reagent (1556018, Invitrogen, USA), SYBR GREEN qPCR Super Mix (C11733046, Invitrogen, USA), fluorescent PCR (1725274, ABI, USA).

Techniques:

Journal: Technology and Health Care

Article Title: A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

doi: 10.3233/thc-218050

Figure Lengend Snippet: Fig. 3. Ang2 and Tie2 were up-regulated by hydroxyapatite bioceramic extract (**P < 0.01, ***P < 0.001).

Article Snippet: Hydroxyapatite bioceramics (synthetized by Beijing Yihuajian science and Trade Co., Ltd., China), endothelial cell culture medium ECM (1001, ScienCell, USA), Potassium phosphate buffer (PBS) (SH30256 01B, Hyclone, USA), clean bench (sw-cj-ifd, Suzhou Antai, China), low-speed centrifuge (sc3614, Zhongjia, China), inverted optical microscope (CKX41, Olympus, Japan), cell incubator (Heracell 150i, Thermo Scientific, USA) and CCK-8 solution (Kga317, Jiangsu Kaiji, China), plate reader (Multiscan MK3, Thermo Fisher Scientific, USA ), Ang1 and Tie2 antibody (251671, Beijing Zen bio, China), Ang2 antibody (24613-1-ap, Proteintech, USA), RIPA buffer (P0013B, Shanghai Beyotime, China), secondary antibody (111-035-003, Jackson ImmunoResearch, USA), luminescent solution (wbkls0500, Millipore, USA), BCA Protein Assay Kit (PA115, Beijing Tiangen, China), PVDF membrane (ipvh00010, Millipore, USA), TRIzol reagent (1556018, Invitrogen, USA), SYBR GREEN qPCR Super Mix (C11733046, Invitrogen, USA), fluorescent PCR (1725274, ABI, USA).

Techniques:

Journal: Technology and Health Care

Article Title: A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

doi: 10.3233/thc-218050

Figure Lengend Snippet: Fig. 4. The transcriptional level of ANG2 and TIE2 were up-regulated by hydroxyapatite bioceramic extract (**P < 0.01, ***P < 0.001).

Article Snippet: Hydroxyapatite bioceramics (synthetized by Beijing Yihuajian science and Trade Co., Ltd., China), endothelial cell culture medium ECM (1001, ScienCell, USA), Potassium phosphate buffer (PBS) (SH30256 01B, Hyclone, USA), clean bench (sw-cj-ifd, Suzhou Antai, China), low-speed centrifuge (sc3614, Zhongjia, China), inverted optical microscope (CKX41, Olympus, Japan), cell incubator (Heracell 150i, Thermo Scientific, USA) and CCK-8 solution (Kga317, Jiangsu Kaiji, China), plate reader (Multiscan MK3, Thermo Fisher Scientific, USA ), Ang1 and Tie2 antibody (251671, Beijing Zen bio, China), Ang2 antibody (24613-1-ap, Proteintech, USA), RIPA buffer (P0013B, Shanghai Beyotime, China), secondary antibody (111-035-003, Jackson ImmunoResearch, USA), luminescent solution (wbkls0500, Millipore, USA), BCA Protein Assay Kit (PA115, Beijing Tiangen, China), PVDF membrane (ipvh00010, Millipore, USA), TRIzol reagent (1556018, Invitrogen, USA), SYBR GREEN qPCR Super Mix (C11733046, Invitrogen, USA), fluorescent PCR (1725274, ABI, USA).

Techniques: